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An enzyme follows Michaelis-Menten kinetics with a Km of 2 mM. When substrate concentration is 2 mM, the reaction velocity is measured at 15 μmol/min. What is the Vmax of this enzyme?
✓ Correct! By the Michaelis-Menten equation, when [S] = Km, v = Vmax/2. Since v = 15 μmol/min at Km, Vmax = 30 μmol/min.
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